Phenotype Image Detail

Image

Caption

Ultrastructural analysis of Snta1^tm1Scf/Snta1^tm1Scf neuromuscular junctions. Nerve-muscle contacts in Snta1^tm1Scf/Snta1^tm1Scf (a-Syn^-/-, A and B) and wild-type (a-Syn^+/+, D) sternomastoid muscles treated with tannic acid to highlight synaptic clefts and junctional folds (synaptic basal lamina). Folds in mutant neuromuscular juntions (NMJs) varied from near normal in appearance (straight, oriented toward the membrane; B) to moderately deranged (curved elements, short folds, folds parallel to the membrane; A). The number of junctional fold openings to the synaptic cleft was reduced in mutant NMJs. Arrows in A and B indicate the only folds that open to the cleft in the views shown. In contrast, 12 such folds are apparent in the view of the wild-type NMJs (D). Pooled data for junctional fold openings per micrometer of presynaptic membrane in wild-type and mutant NMJs are shown in C. The small vesicle-like structures (arrowhead in A), which appear to be caveoli budding from the junctional folds, were plentiful in all NMJs, although few are present in the wild-type image shown (D). Bar: A, 1.1um; B, 1.5um; D, 1.4um.

Copyright

This image is from Adams ME, J Cell Biol 2000 Sep 18;150(6):1385-97, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:76455

Associated Alleles

Symbol	Name
Snta1^tm1Scf	syntrophin, acidic 1; targeted mutation 1, Stanley C Froehner

Associated Genotypes

Allelic Composition	Genetic Background
Snta1^tm1Scf/Snta1^tm1Scf	involves: 129P2/OlaHsd * C57BL/6