Phenotype Image Detail

Image

Caption

Phenotypic analysis of Itgb1^tm1Ehi/Itgb1^tm1Ehi embryos. A-D: Whole-mount wild-type (WT/WT, A and C) and Itgb1^tm1Ehi/Itgb1^tm1Ehi (B1de6/B1de6, B and D) embryos are shown at 9.5 (A and B) and 10.5 (C and D) dpc. B: Mutant embryos displaying defective placentation. D: Mutant embryos showing normal chorion-allantois fusion but cardiac defects. A reduction of the anterior-posterior axis and a significant disconnection between myocardium and preicardium is visible. E-G: Histological analysis of 9.5 dpc embryos. E: Wild-type embryo. F and G: Mutant embryo showing lack of chorion-allantois fusion. Note that allantois and chorion are located on distinct planes and oppositely oriented. H: Transverse section of the head of a mutant embryo at 10.5 dpc showing hindbrain exencephaly and lack of chorion-allantois fusion. I: Section of the posterior trunk of a 10.5 dpc wild-type embryo. Somites already show differentiated dermomyotome and sclerotome. J: Section of the back of a corresponding mutant embryo, showing the retarded somite development and subsequent reduction of mesenchymal cells. Of note is the presence of hemorrhage in the exocoelom. a, atrium; al, allantois; am, amnion; b, primordial blood cells; ba, branchial arch; c, chorion; cn, caudal neuropore; da, dorsal aorta; sm, demomyotome component of somite; f, pharynx; h, heart; ivf, inter-ventricular foramen; mc, myelocoel; om, open myelencephalon; p, placenta; pc, pericardium; s, somites; sc, spinal cord; st, sclerotome component of somite; t, telencephalon; tv, telencephalic vesicle; u, umbilical stalk; uv, umbilical vessels; v, ventricle; ys, yolk sac. Bars: A-H, 1 mm; I, 200 um.

Copyright

This image is from Hirsch E, J Cell Biol 2002 Apr 29;157(3):481-92, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:76216

Associated Alleles

Symbol	Name
Itgb1^tm1Ehi	integrin beta 1 (fibronectin receptor beta); targeted mutation 1, Emilio Hirsch

Associated Genotypes

Allelic Composition	Genetic Background
Itgb1^tm1Ehi/Itgb1^tm1Ehi	either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)