Phenotype Image Detail

Image

Caption

Histology of molars and incisors and characterization of multilayered cells of Ambn^tm1Nid/Ambn^tm1Nid mice. A: Hematoxylin and eosin staining of top first molars of E18, P1, P3 and P7 Ambn^tm1Nid/Ambn⁺ (+/-, top) and Ambn^tm1Nid/Ambn^tm1Nid (-/-, bottom) mice. Loss of cell polarity and detachment from the matrix were observed in P3. P7 molar ameloblast cells accumulate and fail to form columnar shaped single cell layers. B: Incisors in 6 week old heterozygous (1) and homozygous mutant (2) mice. High magnifications of the presecretory and secretory stages (3) and the early maturation stage (4) of mutant incisors. Homozygous ameloblasts from multiple layers containing abnormal calcified structures (arrows). Connective tissue (CT) was migrated into the detached space between ameloblasts and the matrix surface. Am, ameloblasts; D, dentin; EO, enamel organ; Od, odontoblasts; PS, presecretory stage; PD, predentin; P, pulp; E, enamel layer; arrows, calcified structures, dE, defective enamel. C: Immunostaining of P7 normal (top) and homozygous mutant molars (bottom) with anti-amelogenin, enamelin, and tuftelin antibodies and Cy-3-conjugated secondary antibody. DAPI staining was used for nuclear localization. Dashed lines show the border between ameloblasts and the stratum intermedium interface (top) and margin of multilayered cells (bottom). Arrows indicate abnormal matrix accumulating enamel matrix proteins. D: PCNA immunostaining of ameloblasts (right) and nuclear staining with Hoechst dye (left). am, ameloblast; si, stratum intermedium; en, enamel; sr, stellate reticulum. Bars, 25 um.

Copyright

This image is from Fukumoto S, J Cell Biol 2004 Dec 6;167(5):973-83, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:94844

Associated Alleles

Symbol	Name
Ambn^tm1Nid	ameloblastin; targeted mutation 1, Yoshihiko Yamada

Associated Genotypes

Allelic Composition	Genetic Background
Ambn^tm1Nid/Ambn^tm1Nid	involves: 129S1/Sv * 129X1/SvJ * C57BL/6