Phenotype Image Detail

Image

Caption

A: Tumor appearance in 36 week old Ambn^tm1Nid/Ambn^tm1Nid mouse. Tumors were observed only on the right side of the maxillar of mutant mice. Right panel shows micro X-ray radiographs by Faxitron. Arrow and arrowheads indicate tumor. B: Frequency of tumor formation with age. The earliest tumor was observed at 26 weeks old. Only one heterozygous mouse developed a tumor. C: Hematoxylin and eosin staining of tumor lesion of 36 week old mutant mouse shown in A. Dotted lines indicate tumor lesions and the boxed area is enlarged at the bottom panel. D: Immunostaining of tumor lesions. Top panel shows immunostaining of focal area and bottom panel shows surrounding cells. The focal area is stained with antibodies to enamel matrix proteins except ameloblastin, indicating accumulation of enamel matrix proteins. Cells surrounding focal areas are immunopositive for enamel matrix proteins, suggesting ameloblast origin. AMBN, ameloblastin; AMEL, amelogenin; ENAM, enamelin; int alpha6, integrin alpha6. E: RT-PCR analysis of RNA from tumor lesions. RNA samples for lanes +/- and -/- were from dental epithelium of P7 molar of homozygous and heterozygous mice, respectively. RNA of lane T3 was from a 51 week old heterozygous mouse. Note: heterozgyous mouse did not express ameloblastin mRNA in the tumor but expressed mRNA for other enamel matrix proteins similar to the homozygous mice.

Copyright

This image is from Fukumoto S, J Cell Biol 2004 Dec 6;167(5):973-83, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:94844

Associated Alleles

Symbol	Name
Ambn^tm1Nid	ameloblastin; targeted mutation 1, Yoshihiko Yamada

Associated Genotypes

Allelic Composition	Genetic Background
Ambn^tm1Nid/Ambn^tm1Nid	involves: 129S1/Sv * 129X1/SvJ * C57BL/6