Phenotype Image Detail

Image

Caption

Analysis of synaptonemal complex (SC) formation in Sycp2^tm1Jw/Sycp2^tm1Jw spermatocytes. Surface-spread nuclei were stained with anti-SYCP3 antibodies, CREST and DAPl, or they were stained with silver nitrate. A: SYCP3 labels SCs in wild-type (+/+) spermatocytes. (B) SYCP3 fails to incorporate into SCs in Sycp2^tm1Jw/Sycp2^tm1Jw (-/-) spermatocytes. SYCP3 forms several large protein aggregates (arrows). In addition, SYCP3 also forms many small nuclear foci (arrowhead). C: SCs in wild-type spermatocytes are readily stained with silver nitrate. D: No silver-stained SC structures are visible by light microscopy in mutant spermatocytes. E: EM analysis of a wild-type spermatocyte. A tripartite structure of SC is formed by one CE (arrow) that connects with two electron-dense lateral elements (LEs). This SC is attached to the nuclear envelope (NE). Note the condensed chromatin around the SC. F: EM analysis of a mutant spermatocyte. Transverse filaments (TFs) form a central element (CE)-like structure (arrow). Two rows of chromatin grains align in parallel with the CE-like structure to form a SC-like structure. Bars, 0.2 m.

Copyright

This image is from Yang F, J Cell Biol 2006 May 22;173(4):497-507, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:111287

Associated Alleles

Symbol	Name
Sycp2^tm1Jw	synaptonemal complex protein 2; targeted mutation 1, Jeremy Wang

Associated Genotypes

Allelic Composition	Genetic Background
Sycp2^tm1Jw/Sycp2^tm1Jw	involves: 129S4/SvJae * C57BL/6