Phenotype Image Detail

Image

Caption

Kidneys of Cd151^tm1Nki/Cd151^tm1Nki (Cd151^-/-) and Itga3^tm1Son/Itga3^tm1Son Tg(NPHS2-cre)295Lbh/0 (2.5P-Cre; Itga3^fl/fl) mice reveal mild (left column) and severe (middle and right columns) renal pathology. Although the mildly affected kidneys of a 3 month old Cd151^tm1Nki/Cd151^tm1Nki mouse show a relatively smooth and intact surface (A), histological examination reveals protein casts (D, arrow), inconspicuous interstitial fibrosis, mesangial hypercellularity (G, open arrowhead), and mild glomerular sclerosis (G, open arrow). The glomerular basement membrane (GBM) shows partial thickening and spike formation (J, inset). Severe lesions of another 3 month old Cd151^tm1Nki/Cd151^tm1Nki mouse are characterized by a granular surface (B) caused by extensive glomerular collapsing sclerosis, periglomerular fibrosis, and focal interstitial inflammation and fibrosis (E, H, and K). The glomeruli show segmented (H, 1) and global (H, 2) attachment of glomerular tufts to Bowman's capsule, parietal epithelial crescents (H, 2), and hypercellularity, capillary collapse, and sclerosis (H, 3). Proximal tubuli show cystic dilation (E, arrowheads) and degeneration, as well as protein casts indicative of proteinuria (E and H, arrows). Silver staining indicates excessive GBM damage in some glomeruli (K). Severely affected kidneys of a 6 week old Itga3^tm1Son/Itga3^tm1Son Tg(NPHS2-cre)295Lbh/0 mouse reveal prominent protein casts and some extremely dilated proximal tubuli (F and I, arrows). The glomeruli are partially sclerosed and show substantial damage of the GBM (L). Bars, 50 um.

Copyright

This image is from Sachs N, J Cell Biol 2006 Oct 9;175(1):33-9, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:114988

Associated Alleles

Symbol	Name
Cd151^tm1Nki	CD151 antigen; targeted mutation 1, The Netherlands Cancer Institute
Itga3^tm1Son	integrin alpha 3; targeted mutation 1, Arnaud Sonnenberg
Tg(NPHS2-cre)295Lbh	transgene insertion 295, Lawrence B Holzman

Associated Genotypes

Allelic Composition	Genetic Background
Cd151^tm1Nki/Cd151^tm1Nki	involves: 129P2/OlaHsd * FVB/N
Itga3^tm1Son/Itga3^tm1Son Tg(NPHS2-cre)295Lbh/0	Not Specified