Phenotype Image Detail

Image

Caption

Electron micrographs reveal glomerular basement membrane abnormalities and foot process effacement in kidneys of mildly affected Cd151^tm1Nki/Cd151^tm1Nki (Cd151^-/-) and Itga3^tm1Son/Itga3^tm1Son Tg(NPHS2-cre)295Lbh/0 (2.5P-Cre; Itga3^fl/fl), but not Itga3^tm1Son/Itga3⁺ Tg(NPHS2-cre)295Lbh/0 (2.5P-Cre; Itga3^fl/+) mice. A: Glomeruli of 3 month old Cd151^tm1Nki/Cd151^tm1Nki mice show abnormal capillary loops characterized by glomerular basement membrane (GBM) thickening and loss of regular pattern of podocyte foot processes (FPs). B: Peripheral glomerular capillary with a luminal thrombocyte and loss of fenestrations (arrowhead). The GBM is irregularly thickened and shows protrusions toward the capsular space (*). Podocyte FPs are partially lost (arrows). C: Irregular GBM showing lamination, thickening and protrusions (*). The endothelium is swollen and fenestrations are partially lost (arrowheads), as are podocyte FPs (arrows). Glomeruli of 6 week old Itga3^tm1Son/Itga3⁺ Tg(NPHS2-cre)295Lbh/0 (2.5P-Cre; Itga3^+/fl) mice do not show abnormalities (D), whereas all glomeruli of Itga3^tm1Son/Itga3^tm1Son Tg(NPHS2-cre)295Lbh/0 (2.5P-Cre; Itga3^fl/fl) mice exhibit severe structural changes (E and F). E: Abnormally thickened GBM with protusions throughout the glomerulus. F: Podocyte FPs are completely lost (arrows) and GBM protusions (*) are present along the entire length of the capillary loops. Solid lines point to normal structures (GBM and FPs), whereas dotted lines indicate abnormal thickening of the GBM and effacement of FPs. Bc, Bowman's capsule; Cs, capsular space; E, erythrocyte; Ec, endothelial cell; Fp, foot process; Lb, lamina basalis (GBM); Pdc, podocyte; T, thrombocyte; V, vascular lumen. Bars: A, D and E, 10 um; B, C and F, 2 um.

Copyright

This image is from Sachs N, J Cell Biol 2006 Oct 9;175(1):33-9, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:114988

Associated Alleles

Symbol	Name
Cd151^tm1Nki	CD151 antigen; targeted mutation 1, The Netherlands Cancer Institute
Itga3^tm1Son	integrin alpha 3; targeted mutation 1, Arnaud Sonnenberg
Tg(NPHS2-cre)295Lbh	transgene insertion 295, Lawrence B Holzman

Associated Genotypes

Allelic Composition	Genetic Background
Cd151^tm1Nki/Cd151^tm1Nki	involves: 129P2/OlaHsd * FVB/N
Itga3^tm1Son/Itga3^tm1Son Tg(NPHS2-cre)295Lbh/0	Not Specified