Phenotype Image Detail

Image

Caption

A-D: Ki-67 immunofluorescence (red; nuclei are shown in blue) reveals localization to intestinal epithelial cell (IEC) nuclei in the crypt bases (small arrows) in the jejunum of P6 control (WT) mice (A). Increased nuclear Ki-67 detection in the crypt (small arrows) and villous (large arrows) jejunal IECs of P6 Itgb1^tm1Efu/Itgb1^tm1Efu Tg(Vil1-cre)997Gum/0 (KO) mice (B and C). (D) Zoomed view of inset in C shows ectopic foci of Ki-67-positive nuclei in the villi. E and F: Immunohistochemical detection of Musashi-1 in the jejunal crypts (outlined by black dashed lines) of P6 control (E) and mutant (F) mice. G and H: Immunohistochemical detection of Tcf-4 (brown) in nuclei of crypts (black arrows) and villous (red arrows) IECs in the jejunum of P6 control (G) and mutant (H) mice. I: Tcf-4 Western blot of nuclear lysates of jejunal IECs from P6 control and mutant mice. All lanes contain 100 g of total protein. J: Phospho-ERK and ERK-1 Western blots performed on jejunal IEC lysates from P6 control and mutant mice. The white and black arrowheads show p44 and p42 ERKs, respectively, in the top blot. The white arrowhead shows p-ERK, and the black arrowhead shows unphosphorylated ERK in the bottom blot. Bars: A-C, 0.1 mm; D-H, 0.05 mm.

Copyright

This image is from Jones RG, J Cell Biol 2006 Nov 6;175(3):505-14, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:119155

Associated Alleles

Symbol	Name
Itgb1^tm1Efu	integrin beta 1 (fibronectin receptor beta); targeted mutation 1, Elaine Fuchs
Tg(Vil1-cre)997Gum	transgene insertion 997, Deborah L Gumucio

Associated Genotypes

Allelic Composition	Genetic Background
Itgb1^tm1Efu/Itgb1^tm1Efu Tg(Vil1-cre)997Gum/0	involves: 129X1/SvJ * C57BL/6J * SJL