Phenotype Image Detail

Image

Caption

Fertility defect and meiotic progression in Rad51c^tm1Sks/Rad51c^tm1.1Sks mutant females. A-F: Histology of ovaries showing an ovulation defect in infertile Rad51c^tm1Sks/Rad51c^tm1.1Sks (Rad51c^ko/neo) females. In contrast to control females (A and B), no corpora lutea (CL) were seen in ovaries from infertile mutant females at 6 (D) or 12 (E) weeks of age. However, successful ovulation was achieved after hormone treatment from control (C) and mutant (F) ovaries. Note that there are still fewer corpora lutea in mutant ovaries after the superovulation procedure (F). Bars, 200 um. G and H: Infertile mutant females produce developmentally abnormal embryos after superovulation. Only one out of five embryonic day 8.5 embryos produced by an infertile mutant female (H) appeared comparable with a control embryo (G). I-K: Large portion of oocytes from mutant females revealed improperly aligned chromosomes at metaphase I (J) after 8 hours of in vitro maturation compared with control heterozygous littermates (I). Some oocytes from mutant females do show a normal alignment of chromosomes at the metaphase plate (K). The chromosomes were stained by DAPI. For better visualization, the blue DAPI staining has been converted to red. The green signal represents the spindle microtubules.

Copyright

This image is from Kuznetsov S, J Cell Biol 2007 Feb 26;176(5):581-92, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:119835

Associated Alleles

Symbol	Name
Rad51c^tm1.1Sks	RAD51 paralog C; targeted mutation 1.1, Shyam K Sharan
Rad51c^tm1Sks	RAD51 paralog C; targeted mutation 1, Shyam K Sharan

Associated Genotypes

Allelic Composition	Genetic Background
Rad51c^tm1Sks/Rad51c^tm1.1Sks	involves: 129/Sv * C57BL/6J * FVB/N