Phenotype Image Detail

Image

Caption

Ultrastructural anomalies in Alox12b^tm1.1Krie/Alox12b^tm1.1Krie mice. A-C: Methylene blue staining images of semithin sections from newborn dorsal skin of wild-type (+/+, A) and Alox12b^tm1.1Krie/Alox12b^tm1.1Krie (-/-, B and C) mice. Arrows indicate irregular vesicular structures in the upper granular layers of the mutant skin, D-L: Back skin of wild-type (D) and mutant (E-L) mice was processed from transmission EM. D: Normal horny and granular layer in wild-type epidermis. Asterisks depict keratohyalin granules. E: Abnormal vesicular structures (asterisks) within granular layer. F: Normal lamellar bodies (arrows) within granular layer. G: Normal lamellar body; improved presentation of lamellae stacks by ruthenium tetroxide postfixation. H: Improved visualization of lipid lamellae stacks between uppermost epidermal cell and first keratinized cell. I and J: Membranous material resembling the content of lamellar bodies adheres to the marginal membrane of irregular vesicles (arrows). K and L: Details of irregular vesicles; membranous material and small vesicle. Bars: A-C, 10 um; D, 2.25 um; E, 3 um; F, 500 nm; G and H, 50 nm; I and J, 513/250 nm; K and L, 500/200 nm.

Copyright

This image is from Epp N, J Cell Biol 2007 Apr 9;177(1):173-82, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:134709

Associated Alleles

Symbol	Name
Alox12b^tm1.1Krie	arachidonate 12-lipoxygenase, 12R type; targeted mutation 1.1, Peter Krieg

Associated Genotypes

Allelic Composition	Genetic Background
Alox12b^tm1.1Krie/Alox12b^tm1.1Krie	involves: 129P2/OlaHsd * 129S6/SvEvTac * BALB/cJ * C57BL/6