Phenotype Image Detail

Image

Caption

A-C: Toluidine blue-stained semithin sections of distal limb from control (A) and Lama5^tm1Jhm/Lama5^tm1Jhm (B and C) E14.5 embryos. The surface ectoderm (se) is continuous in controls (A). In mutants, in contrast, the ectoderm had been breached, and extruded mesenchymal cells (m) have migrated along the outer surface of the limb (B). A nearby section from the same mutant limb shows a thickened surface ectoderm (*) between the outer and inner mesenchymal populations (C). D: Immunostaining of mutant limb with an antibody to laminin gamma 1 demonstrates the presence of the basal lamina (BL) material on both sides of the thickened ectoderm (arrowheads), suggesting that the ectoderm has maintained a proper relationship with the displaced mesenchymal cells. E and F: Ultrastructural analysis of distal limb BL at E14.5 shows a dense, continuous BL (arrowhead) in the control (E) but a patchy, discontinuous BL in the mutant (F). Bars: A-C, 62.5 um; D, 50 um; E and F, 0.25 um.

Copyright

This image is from Miner JH, J Cell Biol 1998 Dec 14;143(6):1713-23, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:51557

Associated Alleles

Symbol	Name
Lama5^tm1Jhm	laminin, alpha 5; targeted mutation 1, Jeffrey H Miner

Associated Genotypes

Allelic Composition	Genetic Background
Lama5^tm1Jhm/Lama5^tm1Jhm	involves: 129 * C57BL/6J