Phenotype Image Detail

Image

Caption

A and B: Whole mount views of control and Lama5^tm1Jhm/Lama5^tm1Jhm embryos at E13.5. Exencephalics lack the skin and skull that normally enclose the brain and have a topologically "inside out" brain that does not develop properly. The skin and skull of the control was dissected away to allow direct comparison of mutant and normal brain. C and D: Immunohistochemical localization of proliferating cells with anti-BrdU and alkaline phosphatase chemistry after labeling for 1 hour in utero with BrdU. C: In normal neural tissue (n), BrdU-labeled cells (arrow) are confined to the ventricular zone. V, ventricle. D: In exencephalics, labeled cells (arrow) are found on the outer surface of the neural tissue. This surface would have been ventricular had the neural tube closed, but now it is in direct contact with amniotic fluid. Skin and skull (s) overlay the brain in the control but are missing from the mutant. Pia is indicated by dashed lines. E and F: Toulidine blue-stained sections through the anterior of E8.7 control and mutant embryos; the neural tube is unclosed at this stage in both control and mutant. Bars: B, 1 mm; D, 0.25mm; F, 0.2 mm.

Copyright

This image is from Miner JH, J Cell Biol 1998 Dec 14;143(6):1713-23, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:51557

Associated Alleles

Symbol	Name
Lama5^tm1Jhm	laminin, alpha 5; targeted mutation 1, Jeffrey H Miner

Associated Genotypes

Allelic Composition	Genetic Background
Lama5^tm1Jhm/Lama5^tm1Jhm	involves: 129 * C57BL/6J