Phenotype Image Detail

Image

Caption

Frozen sections through growth plates of distal radii of wild-type (WT, Left) and Bpnt2^{Gt(RST634)Byg}/Bpnt2^{Gt(RST634)Byg} (gt/gt, Right) E18.5 mice stained with Alcian blue (AB), pH 1.0 to visualize cartilaginous tissue, von Kossa reagent for calcified bone (brown), and NFR. The overall reduced length of the mutant growth plate with particularly shortened zones of epiphyseal chondrocytes (EC) and columnar chrondrocyte (CC) is demonstrated. Delineation of EC, CC and hypertrophic chondrocytes (HC) zones are indicated by solid arcs. Dashed boxes within EC, CC and HC zones represent the areas of the growth plate shown magnified in i, ii and iii, respectively. Relatively increased amounts of AB-positive ECM surrounding mutant chondrocytes is depicted in all three zones with large regions of acellular space particularly evident around CCs and HCs (ii and iii; *). The normally longitudinal orientation of chondrocyte columns, as shown in the wild-type growth plate, is disrupted in the mutant growth plate (ii). The medial to lateral orientation of the mutant CCs corresponds with a flared metaphysis (arrow). Reduced size of mutant columnar and hypertrophic chondrocytes is shown in ii and iii. Scale bars: 50 microns.

Copyright

This image is from Frederick JP, Proc Natl Acad Sci U S A 2008 Aug 19;105(33):11605-12. Copyright 2008 National Academy of Sciences, U.S.A. J:139706

Associated Alleles

Symbol	Name
Bpnt2^Gt(RST634)Byg	3'(2'), 5'-bisphosphate nucleotidase 2; gene trap RST634, BayGenomics

Associated Genotypes

Allelic Composition	Genetic Background
Bpnt2^Gt(RST634)Byg/Bpnt2^Gt(RST634)Byg	involves: 129P2/OlaHsd * C57BL/6