Phenotype Image Detail

Image

Caption

Proliferation assay of wild-type and Pik3ca^tm1Nbm/Pik3ca^tm1Nbm embryos. Sagittal sections of E9.5 wild-type (panels A-C) and Pik3ca^tm1Nbm/Pik3ca^tm1Nbm (panels D-F) embryos harvested 1 hour after intraperitoneal injection of the pregnant mouse with BrdUrd. A and D are from the midbrain region, B and E are from region of rhombomere, and C and F are from neural tube. Heavy brown staining of most cells in A-C from wild-type embryos indicates that proliferation (as evidenced by BrdUrd incorporation) was taking place. The nearly complete absence of similar staiing in the mutant embryos shows that despite generally normal development up to E9.5, embryonic cells had ceased to proliferate.

Copyright

This image is from Bi L, J Biol Chem 1999 Apr 16;274(16):10963-8 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:54237

Associated Alleles

Symbol	Name
Pik3ca^tm1Nbm	phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; targeted mutation 1, Robert L Nussbaum

Associated Genotypes

Allelic Composition	Genetic Background
Pik3ca^tm1Nbm/Pik3ca^tm1Nbm	involves: 129S6/SvEvTac