Phenotype Image Detail

Image

Caption

Transmission electron micrographs of embryonic day 18 kidneys from wild-type and Podxl^tm1Kmn/Podxl^tm1Kmn embryos. A: Wild-type (+/+) kidneys have normal podocytes (Pod.) with typical major processes (MPs) and foot processes (FPs) that embrace the outer aspect of the capillary basement membrane. Red blood cells (RBCs) can be seen in the lumen of the endothelial vessels. Podxl^tm1Kmn/Podxl^tm1Kmn (-/-) mice show a complete loss of slit diaphragms (SDs) and FPs. Junctional complexes (JCs) are present between all of the mutant podocytes. Podocytes in mutant mice also display numerous vacuoles (Vac.) and overall the glomerular capillaries had a thicker endothelial cell (EC) layer. Scale bars, 2 um. B: Higher magnification reveals that the SDs and FPs indicated by arrows in the wild-type and Podxl^tm1Kmn/Podxl⁺ (+/-) mice are completely lost in homozygous mutant glomeruli. JCs including tight junctions (TJs) and adherens junctions (AJs) are present between homozygous mutant podocytes. The endothelial fenestrae (F) within the ECs can be found in wild-type controls but the fenestrae reduced in the homozygous mutant glomerular capillaries. Scale bars, 0.2 um.

Copyright

This image is from Doyonnas R, J Exp Med 2001 Jul 2;194(1):13-27, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:70294

Associated Alleles

Symbol	Name
Podxl^tm1Kmn	podocalyxin-like; targeted mutation 1, Kelly M McNagny

Associated Genotypes

Allelic Composition	Genetic Background
Podxl^tm1Kmn/Podxl^tm1Kmn	either: (involves: 129P2/OlaHsd * BALB/c) or (involves: 129P2/OlsHsd * C57BL/6)
Podxl^tm1Kmn/Podxl^+	either: (involves: 129P2/OlaHsd * BALB/c) or (involves: 129P2/OlsHsd * C57BL/6)