Phenotype Image Detail

Image

Caption

Cranial and trunk mesenchyme deficiencies in Prkar1a^tm1Gsm/Prkar1a^tm1Gsm embryos. A: Scanning electron microscopy of the head of an E9.5 wild-type embryo. Note the well developed forebrain, midbrain and hindbrain resulting from complete closure of the neural tube. The first and second branchial arches are present and lie adjacent to the pericardium. The otic vesicle has delaminated from the surface ectoderm, and the optic vesicle is visible. B: Scanning electron microscopy of the head of an E9.5 Prkar1a^tm1Gsm/Prkar1a^tm1Gsm embryo. Note the open neural tube in the forebrain and midbrain region. Branchial arches are absent, and cranial mesenchyme is severely deficient. The otic vesicle is present and has delaminated from the surface ectoderm. C: E8.5 wild-type embryo showing ventral heart tube formation, including both endocardial and myocardial elements suspended within the pericardium and attached via the dorsal mesocardium. D: Equivalent section through an E9.5 mutant embryo showing the absence of a ventral linear heart tube and pericardium. Precardiac mesoderm is present on either side of the midline of the pharynx. Note the persistence of the intraembryonic coelomic cavities that have failed to fuse and form the pericardium. E: Whole mount in situ staining of an E9.5 mutant and an E9.5 wild-type embryo using a Pax3 antisense riboprobe. Note the staining in the 10-12 tiny somites that lie adjacent to the neural tube. Somites in the mutant are segmented but are greatly reduced in size and number when compared with the E9.5 wild-type littermate. F, ventral view of E9.5 mutant compared with an E9.5 wild-type littermate. Note the absence of fusion of somites across the midline, indicating the presence of notochord tissue. Scale bars in A-D = 100 m; E and F = 500 m. The abbreviations used are as follows: am, amnion; cm, cardiomyocytes; co, intraembryonic coelomic cavity; da, dorsal aorta; en, endocardium; fg, foregut; hp, head process; mc, myocardium; ng, neural groove; pcm, precardiac mesoderm; ph, pharynx; ysm, yolk sac membrane.

Copyright

This image is from Amieux PS, J Biol Chem 2002 Jul 26;277(30):27294-304 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:78010

Associated Alleles

Symbol	Name
Prkar1a^tm1Gsm	protein kinase, cAMP dependent regulatory, type I, alpha; targeted mutation 1, G Stanley McKnight

Associated Genotypes

Allelic Composition	Genetic Background
Prkar1a^tm1Gsm/Prkar1a^tm1Gsm	involves: 129X1/SvJ * C57BL/6