Phenotype Image Detail

Image

Caption

Disturbed epidermal barrier in Slc27a4^tm1Wsr/Slc27a4^tm1Wsr mice. A: Diffusion of Lucifer yellow in neonatal mice. The fluorescent dye Lucifer yellow did not pass the upper layers of the stratum corneum in wild-type (Fatp4 +/+) neonatal mice. In contrast, in Slc27a4^tm1Wsr/Slc27a4^tm1Wsr (Fatp4 -/-) pups, it permeated the entire epidermis. Bars, 50 um. B: Access of X-gal to skin of embryonic day 18.5 mice. Slc27a4^tm1Wsr/Slc27a4⁺ (Fatp4 +/-) epidermis is impermeable of X-gal. In contrast, X-gal permeates mutant skin, where it is cleaved by endogenous beta-galactosidase activity to produce a colored precipitate. A heterozygous mouse is shown as a control to exclude false-positive staining due to beta-galactosidase encoded by the lacZ gene of the mutant Slc27a4 allele.

Copyright

This image is from Herrmann T, J Cell Biol 2003 Jun 23;161(6):1105-15, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:84096

Associated Alleles

Symbol	Name
Slc27a4^tm1Wsr	solute carrier family 27 (fatty acid transporter), member 4; targeted mutation 1, Wolfgang Stremmel

Associated Genotypes

Allelic Composition	Genetic Background
Slc27a4^tm1Wsr/Slc27a4^tm1Wsr	involves: 129P2/OlaHsd * C57BL/6