Phenotype Image Detail

Image

Caption

A: Histological sectioning of long bone of postnatal mouse day 1 (D1) and day 5 (D5) shows normal organization of the epiphysis and the growth plate and mineralization of bone tissue. By day 5 fewer trabeculae were in the Wwox^tm1Ria/Wwox^tm1Ria (KO) mouse compared with wild-type (WT) and Wwox^tm1Ria/Wwox⁺ (HT) (bracket). Sections were stained with toluidine blue and von Kossa silver stain (for detection of mineralized tissues; black). B: uCT image of the metaphysis region of wild-type and mutant femur at day 15 showing less volume of trabecular (Trab.) bone with accompanying quantitation of trabecular bone loss. Ht, heterozygous; Conn Dens, connectivity density; Tiss Dens; tissue density. C: High rate of bone resorption in mutant mice is evident by tartrate-resistant acid phophatase histochemical staining (red) with fast green counterstain of femurs from wid-type and mutant mice. Note increased osteoclast activity in the primary spongiosa under the growth plate on day 3 and osteoclasts on nearly all trabeculae on day 5, consistent with decreased trabeculae number on day 15 in B. Inset in mutant panel shows x63 magnification of osteoclast. On day 5, asterisk shows bone spicules in wild-type without osteoclasts.

Copyright

This image is from Aqeilan RI, J Biol Chem 2008 Aug 1;283(31):21629-39 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:138140

Associated Alleles

Symbol	Name
Wwox^tm1Ria	WW domain-containing oxidoreductase; targeted mutation 1, Rami I Aqeilan

Associated Genotypes

Allelic Composition	Genetic Background
Wwox^tm1Ria/Wwox^tm1Ria	involves: 129X1/SvJ * C57BL/6J