Phenotype Image Detail

Image

Caption

A: Newborn Itgb4^tm1Efu/Itgb4^tm1Efu mouse. Note gross denuding of the body trunk skin. This occurred at birth of all homozygous mice, and it resembled denuding seen in junctional epidermolysis bullosa in humans. B-C: Hematoxylin and eosin stained sections of skin from wild-type (B) and mutant (C) mice. In C, note (1) gross separation at the dermo-epidermal junction (asterisk); (2) pearl-like or organization of some groups of cells in spinous layers (bracket); (3) general disorganization of cells in basal and spinous layers; and (4) the difference in thickness of the stratum corneum in B and C is not real; the upper layers frequently detach during sectioning. D-H: Newborn wild-type (D and F) mutant (E,G and H) skin sections stained with antibodies against K5 (D and E), K6 (F and G), or K10 (green) and K5 (red) (H). Note discontinuous layer of anti-K5 positive cells in E, presumably due to degeneration of basal cells. B-C were taken with a 63x objective and (D-G) were taken with a 40x objective. Abbreviations: BL, basal layer; SP, spinous layer; GR, granular layer; SC, stratum corneum; De, dermis; white dotted line, dermo-epidermal junction; white line, a small blister separating the epidermis from the underlying dermis. White arrowheads point to stratum corneum; white asterisk in E, G and H highlight a pearl of suprabasal keratinocytes displaying basal-like properties.

Copyright

This image is from Dowling J, J Cell Biol 1996 Jul;134(2):559-72, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:77644

Associated Alleles

Symbol	Name
Itgb4^tm1Efu	integrin beta 4; targeted mutation 1, Elaine Fuchs

Associated Genotypes

Allelic Composition	Genetic Background
Itgb4^tm1Efu/Itgb4^tm1Efu	involves: 129S1/Sv * 129X1/SvJ * C57BL/6