Phenotype Image Detail

Image

Caption

Gross morphology of Trip11^{Gt(AJ0490)Wtsi}/Trip11^{Gt(AJ0490)Wtsi} mutants. A: Image of a pair of E18.5 embryos. Note the open mouth and omphalocele (arrow) on the Trip11^{Gt(AJ0490)Wtsi}/Trip11^{Gt(AJ0490)Wtsi} (-/-) embryo. B: Cardiovascular defects in mutant mice. (Ba-Bb, wild-type (+/+)) EFIC images of a wild-type E18.5 embryo showing normal septation of the ventricular chambers (Ba), contrasting with the ventricular septal defect (VSD) seen in mutant embryo shown in (Bc). The aorta (Ao) connects with the left ventricle (LV) (Bb), which contrasts with the mutant embryo shown in (Bf) with an overriding aorta that connects to both ventricles via a VSD. (Bc-Be, mutant) EFIC images of a mutant embryo at E18.5. In the transverse imaging plane (Bc), a large muscular ventricular septal defect (white arrow) can be seen situated posteriorly. Examination in the frontal plane (Bd) showed a second muscular ventricular septal defect (white arrow) positioned anteriorly. Three dimensional reconstruction of the heart (Be) shows the posterior ventricular septal defects (white arrow). (Bf, mutant) Another mutant fetus at E18.5 examined by EFIC imaging was found to have a ventricular septal defect (white arrow) associated with an overriding aorta (Ao). Pulmonary stenosis was indicated with a reduction in the size of the pulmonary outflow (P) compared to the aorta (Ao). There was also apparent thickening of the ventricular chamber walls. This constellation of defects is consistent with Tetralogy of Fallot. At, atrium; LV, left ventricle; RV, right ventricle; S, septum; Cr, cranial; CD, caudal; L, left; R, right; A, anterior; P, posterior. C. Lung. Hematoxylin and eosin staining of E18.5 embryonic lungs. The larger airways are marked with B and the saccules are marked with S. Note the smaller saccule space and thicker inter saccule mesenchyme in the mutant at E18.5. Scale bar is 50um for E18.5 and 100um for E15.5.

Copyright

This image is from Follit JA, PLoS Genet 2008 Dec;4(12):e1000315, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:142875

Associated Alleles

Symbol	Name
Trip11^Gt(AJ0490)Wtsi	thyroid hormone receptor interactor 11; gene trap AJ0490, Wellcome Trust Sanger Institute

Associated Genotypes

Allelic Composition	Genetic Background
Trip11^Gt(AJ0490)Wtsi/Trip11^Gt(AJ0490)Wtsi	involves: 129P2/OlaHsd * C57BL/6