Phenotype Image Detail

Image

Caption

Cells from the three primary layers of the developing heart, namely endocardium, myocardium and epicardium, of E10.5 wild-type (+/+) and Cert1^{Gt(RRF047)Byg}/Cert1^{Gt(RRF047)Byg} (-/-) embryos were examined under an electron microscope. The panels on the left are from the wild-type embryos, and the mutants are on the right. The black arrow shows a lipid-laden engorged rough endoplasmic reticulum (ER, rer) in the endocardial layer of the mutant heart. The white arrows show lipid-laden structures found in the vicinity of swollen ER and mitochondria (m) found often in the mutant cells. The arrowheads show mitochondria undergoing degeneration with discontinuous outer and inner mitochondrial membranes in the mycardial layer (middle). In the epicardial cells (bottom), the arrowheads depict swollen and pale mitochondria with ruptured inner and outer mitochondrial membranes in the mutant heart. The mitochondria in the mutant are generally swollen, pale and have fewer cristae. n, nucleus; mf, muscle fibers of the myocardial cells. Bars, 500 nm.

Copyright

This image is from Wang X, J Cell Biol 2009 Jan 12;184(1):143-58, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:146172

Associated Alleles

Symbol	Name
Cert1^Gt(RRF047)Byg	ceramide transporter 1; gene trap RRF047, BayGenomics

Associated Genotypes

Allelic Composition	Genetic Background
Cert1^Gt(RRF047)Byg/Cert1^Gt(RRF047)Byg	involves: 129P2/OlaHsd * C57BL/6