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Caption | Growth and cell cycle defects without increased apoptosis in Cert1Gt(RRF047)Byg/Cert1Gt(RRF047)Byg (-/-) embryos. A: Pregnant mice were injected with BrdU 1 hour before dissection, and E10.5 embryos were dissected, genotyped, and stained for BrdU uptake. Four of the areas chosen for counting the fraction of dividing cells are shown in the panels to the right. B: Mutant embryos showed a lower fraction of BrdU-positive cells, indicating a slower rate of cell division in these mutant embryos. Error bars indicate standard deviation; n = 4. C: Western blot analysis for some of the components of the cell cycle in wild-type (+/+) and mutant embryos. D: Phosphorylated Akt levels are decreased in mutant embryos. E: TUNEL staining of E10.5 wild-type and mutant embryos. Insets show boxed areas at higher magnification. There were no significant differences in the proportion of dying cells between wild-type and mutant embryos. Note that the wild-type embryos are large compared with the mutant and, therefore, were photographed twice to include the upper and lower halves of the embryo. The composites are shown in A and E. | ||||
Copyright | This image is from Wang X, J Cell Biol 2009 Jan 12;184(1):143-58, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:146172 | ||||
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Associated Genotypes |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 10/29/2024 MGI 6.24 |
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