Phenotype Image Detail

Image

Caption

A: Electron microscopy of transverse sections from the cervial cord of 6 day old wild-type (Nfasc +/+) and Nfasc^tm1.1Brp/Nfasc^tm1.1Brp (Nfasc -/-) mice showed that, at this very early stage of CNS myelination, both myelinating (arrowheads) and nonmyelinated (asterisks) profiles were visible in wild-type and mutant cord. Bar 1 um. B: Western blot analysis of cervical cord homogenates from wild-type and mutant mice showed that the myelin components MAG and MBP were reduced in the mutant at P6 and were comparable to the levels found at P4 in the wild-type. Tubulin was the loading control. All the blots were from the same gel and exposures were identical for each protein. C: The percentage of myelinated axons in cervical cord sections was reduced in the mutant. D: The total number of axons in the optic nerves of wild-type and mutant nerves was not significantly different. E: Oligodendrocyte numbers in wild-type and mutant spinal cord cross sections were not significantly different. Values are means +/- SEM. n=3 mice for each condition. *, P<0.01.

Copyright

This image is from Zonta B, J Cell Biol 2008 Jun 30;181(7):1169-77, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:139185

Associated Alleles

Symbol	Name
Nfasc^tm1.1Brp	neurofascin; targeted mutation 1.1, Peter J Brophy

Associated Genotypes

Allelic Composition	Genetic Background
Nfasc^tm1.1Brp/Nfasc^tm1.1Brp	involves: 129P2/OlaHsd * C57BL/6