Phenotype Image Detail

Image

Caption

Mouse skin samples were taken at 2 days of age, and processed for electron microscopy. (a) Basal layer of Krt14^tm1Efu/Ktr14⁺ and (inset) wild-type (+/+) skin. Basal cells show thick bundles of keratin filaments (Kf) in cytoplasm. (Inset) Keratin filament bundles are attached to hemidesmosomes. Nu, nucleus; BL, basal lamina; Mi, mitochondria; arrowheads, hemidesmosomes. (b) Basal layer of Krt14^tm1Efu/Krt14^tm1Efu mouse skin. Only occasional thin keratin filament bundles (Kf) and some sparse keratin filaments are present. (inset) Desmosome (De) connecting two basal cells. Arrow denotes presence of a few longer filaments attached to these structures. (c) The base of a basal cell from mutant mouse skin. Note presence of an underlying cytoskeleton with filaments (arrows) attached to hemidesmosomes (Hd). (d) Basal and first suprabasal layer of the mutant mouse skin. Note extensive basal cell cytolysis (asterisks) accompanied by degenerated mitochondria (Mi). The suprabasal spinous cell (SP) is still intact, with a normal cytoplasm and thick bundles of keratin filaments (Kf). (e) Suprabasal spinous layers (SP) of mutant mouse skin. Note thick bundles of keratin filaments (Kf) throughout cytoplasm. In upper layers, Kf bundles are attached to keratohyalin granules (Kg). BC, basal cell. Bars: (a-c, inset b) 0.4 um; (a, inset) 0.25 um; (d) 0.3 um; (e) 0.75 um.

Copyright

This image is from Lloyd C, J Cell Biol 1995 Jun;129(5):1329-44, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:25763

Associated Alleles

Symbol	Name
Krt14^tm1Efu	keratin 14; targeted mutation 1, Elaine Fuchs

Associated Genotypes

Allelic Composition	Genetic Background
Krt14^tm1Efu/Krt14^tm1Efu	involves: 129