Phenotype Image Detail

Image

Caption

Ultrastructural analysis of the upper epidermis and cytokeratin filaments in wild-type (a and c) and Krt10^tm1Tmm/Krt10^tm1Tmm (b,d,e) mice. (a) Note the marked morphological changes between the uppermost granular cell (gc) and the lowermost cornified cell (cc). The latter is a flattened anucleated cell with homogenous electron-dense cytoplasm. Organelles and typical type I/II keratohyalin (kh) granules as present in the cell layer below have disappeared completely. (b) In contrast mutant mice show a broad transition zone of multiple layers of incompletely cornified cells. Their cytoplasm contains remnant organelles and shows a dappled pattern of electron-lucent and electron-dense areas. (c) Normal cytokeratin filament bundles (arrowheads) and nuclear membrane (n) are arranged in parallel, separated by a small rim of cytoplasm. The bundle is composed of individual cytokeratin aggregates resulting in a characteristic fibrillar pattern. (d) Abnormal cytokeratin aggregates in suprabasal keratinocytes. Cytokeratin clumps (arrows) are located next to the cell membrane and in association with desmosomes (arrowheads). Note the lack of normal cytokeratin filament bundles in both cells. n, nucleus. (e) Higher magnification reveals that abnormal cytokeratin aggregates (arrows) consist of short cytokeratin filaments in a whorl-like configuration. Note the fibrillar pattern in the periphery and the granular appearance in the center of the cytokeratin aggregates. Asterisk, intercellular space. Bars: (b,c and e) 2 um; (d) 1 um.

Copyright

This image is from Porter RM, J Cell Biol 1996 Mar;132(5):925-36, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:31853

Associated Alleles

Symbol	Name
Krt10^tm1Tmm	keratin 10; targeted mutation 1, Thomas M Magin

Associated Genotypes

Allelic Composition	Genetic Background
Krt10^tm1Tmm/Krt10^tm1Tmm	either: (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * BALB/c * C57BL/6)