Phenotype Image Detail

Image

Caption

Abnormalities in the overall appearance and histopathology of the kidneys of Tns1^tm1Efu/Tns1^tm1Efu mice. A: Wild-type (+/+) and Tns1^tm1Efu/Tns1^tm1Efu (-/-) kidneys at various stages. Shown are representative kidneys from (left to right): 23 wk +/+, 6 wk -/-, 14 wk -/-, 28 wk -/-, and 36 wk -/- mice. Note the pale color of the mutant kidneys at all ages. B: Shown are representative hemisected kidneys from (left to right): 23 wk +/+; 28 wk -/- and 14 wk -/- mice. C-F: Kidneys from 18 wk old mutant mice and their wild-type littermates were placed in formaldehyde fixative, sectioned (5 um), and stained with hematoxylin and eosin. Shown are representative sections from the kidneys of +/+ (C; box denotes region magnified in E) and -/- (D; denotes region magnified in F) mice. Note the enlarged space, corresponding to the renal pelvis, in the mutant (asterisk in D). Note the presence of numerous cysts in the mutant kidney sections, a typical feature of the mutant mice. G-I: Kidneys from 4 week old mutant mice and their wild-type littermates were fixed and stained as outlined above. Shown are representative sections of (G-H) the cortical regions of +/+ (G) and -/- (H) kidneys; note again the prevalence of cysts (open circles) in the mutant kidney. G, glomeruli. I: Section of -/- cortex to illustrate cysts containing amorphous materials (C), often referred to as casts (double arrow in F). J: Section of mutant glomeruli, revealing large space in Bowman's space (double arrow), commonly seen when fluid backs up in the capsule as a result of a malfunctioning of the kidney (see also arrow in F). K: Glomeruli (G) from wild-type kidney; note the normal spacing surrounding Bowman's space. L: Glomeruli from mutant kidney; note that these glomeruli were surrounded by atypical cuboidal epithelial cells with a prominent cytoplasm (arrowheads), rather than the normal thin flattened epithelia, barely visible in the wild-type glomeruli. (Open circles) cysts. M: Glomerulus from a mutant kidney, surrounded by a highly irregular epithelium; also note what appears to be extracellular material, possibly collagen, similar to that seen in focal segment glomerular sclerosis in humans. N: Region from a mutant kidney, depicting marked signs of focal interstitial inflammatory infiltrates. Bar: (C and D) 740 um; (E and F) 400 um; (G and H) 100 um; (I and N) 50 um; (K-M) 25 um; (J) 17 um.

Copyright

This image is from Lo SH, J Cell Biol 1997 Mar 24;136(6):1349-61, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:39202

Associated Alleles

Symbol	Name
Tns1^tm1Efu	tensin 1; targeted mutation 1, Elaine Fuchs

Associated Genotypes

Allelic Composition	Genetic Background
Tns1^tm1Efu/Tns1^tm1Efu	involves: 129S1/Sv * 129X1/SvJ * C57BL/6