Phenotype Image Detail

Image

Caption

Immunoelectron microscopy reveals the presence of focal adhesions in seemingly normal areas of Tns1^tm1Efu/Tns1^tm1Efu kindey tubules and the absence of focal adhesions in cystic tubules. Kidneys from Tns1^tm1Efu/Tns1^tm1Efu mice were processed for regular and immunoelectron microscopy. Shown are ultrathin sections of epithelial cells from the proximal tubules of mildly affected or seemingly unaffected areas (A,C and E) and of severely affected areas (B,D and F). A: Proximal tubule cells from a mildly affected region. Note that the epithelium is polarized, with numerous microvilli on the apical surface. In some regions such as this one, the only abnormality detected was fewer undulations of the basal membrane; however, such differences were not always seen. B: Proximal tubule cells from a severely affected region. Note that only a few residual microvilli remain, and the epithelium shows few signs of polarization. C-F: Tubular epithelial cells labeled with antibodies agains p130 (C and D). Note that the mildly affected epithelial cells still display labeling of focal adhesions (arrows in C). Note the absence of significant labeling in the severely affected regions, suggestive of a loss of focal adhesions. E and F: Tubular epithelial cells labeled with antibodies against the sodium-potassium ATPase (E and F). Note that the mildly affected regions still showed labeling of the basolateral surface, as is typical of wild-type controls (not shown). Note the absence of significant labeling in the severely affected regions, suggestive of a loss of cell polarity. Nu, nucleus, M, mitochondria. Bar: (A) 2.3 um; (B) 1.3 um; (C) 0.6 um; (D) 0.4 um; (E and F) 0.7 um.

Copyright

This image is from Lo SH, J Cell Biol 1997 Mar 24;136(6):1349-61, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:39202

Associated Alleles

Symbol	Name
Tns1^tm1Efu	tensin 1; targeted mutation 1, Elaine Fuchs

Associated Genotypes

Allelic Composition	Genetic Background
Tns1^tm1Efu/Tns1^tm1Efu	involves: 129S1/Sv * 129X1/SvJ * C57BL/6