Phenotype Image Detail

Image

Caption

A: Schematic representation of the mouse placenta (Left) including the labyrinth (lb), the spongiotrophoblast (sp), and the giant cells (gc), adjacent to the maternal decidua (de). In the labyrinth (enlarged view on the Right), maternal blood lacunae, containing maternal red blood cells (mrbc), are separated from fetal blood vessels (fbv), containing fetal red blood cells (frbc), by a trilaminar barrier composed of mononuclear sinusoidal trophoblast giant cell (stgc) and 2 layers of syncytiotrophoblasts (ST-I and ST-II), the latter apposed to the fetal endothelium. B-H: Light microscopy analysis of wild-type (Left) and Syna^tm1.1Ics/Syna^tm1.1Ics (Right) placental tissues. (B and C) Hematoxylin and eosin staining of 5 um placental sections from living E12.5 embryos with the labyrinth, spongiotrophoblast, and maternal decidua delineated. Enlarged view of the labyrinth (C), with fetal and maternal red blood cells indicated. The labyrinth of a mutant placenta is more compact, with the fetal red blood cells squeezed inside the narrowed vessels. D-H: Marker analyses by in situ hybridization for 4311 and mPLI (D and E) or by histochemistry for CD34 (F and G) and Ki67 (H) of placenta sections of E11.5 (D,E and H) and E13.5 (F and G) embryos. CD34-negative nonvascularized areas (in F) are indicated with asterisks; CD34-positive fetal blood vessels (fbv, see enlarged view in G) have a reduced internal space in mutant labyrinth, with fetal erythrocytes (arrowheads) squeezed. H: Ki67-positive cells forming aggregates in the mutant labyrinth. Scale bars: B and D, 400 um; E,F, and H, 100 um; C, 25 um; and G, 12.5 um.

Copyright

This image is from Dupressoir A, Proc Natl Acad Sci U S A 2009 Jul 21;106(29):12127-32. Copyright 2009 National Academy of Sciences, U.S.A. J:150813

Associated Alleles

Symbol	Name
Syna^tm1.1Ics	syncytin a; targeted mutation 1.1, Mouse Clinical Institute

Associated Genotypes

Allelic Composition	Genetic Background
Syna^tm1.1Ics/Syna^tm1.1Ics	involves: 129S2/SvPas * BALB/c * C57BL/6 * SJL