Phenotype Image Detail

Image

Caption

Wild-type (WT) and Mbtps1^tm1Jdh/Mbtps1^tm1Jdh Tg(Col2a1-cre)1Bhr/0 (S1P^cko) forelimb sections were stained with Safranin O, Fast green, and hematoxylin at various time points. The humerus is shown in all panels. Hypertrophic chondrocytes are visible in wild-type mice as early as E13.75 (7 h after E13.5). These hypertrophic cells are structured into a distinct hypertrophic zone that is missing in mutant mice. Endochondral bone is evident in wild-type but absent in mutant mice at E15.5; in the absence of endochondral bone, an exuberant bone collar is formed (arrow). At E18.5, the exuberant bone collar often impinges into the diaphysis and bisects the cartilage. Bar, 200 um. (insets) High magnification pictures showing the morphology of the chondrocytes near the center of the limb at E13.5; the morphology of hypertrophic cells at E13.75; the junction of the proliferating and hypertrophic cells at E14.5; the junction of the bone and hypertrophic cells for wild-type; and the center of the limb for the mutant at E15.5. Bar, 50 um.

Copyright

This image is from Patra D, J Cell Biol 2007 Nov 19;179(4):687-700, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:135374

Associated Alleles

Symbol	Name
Mbtps1^tm1Jdh	membrane-bound transcription factor peptidase, site 1; targeted mutation 1, Jay D Horton
Tg(Col2a1-cre)1Bhr	transgene insertion 1, Richard R Behringer

Associated Genotypes

Allelic Composition	Genetic Background
Mbtps1^tm1Jdh/Mbtps1^tm1Jdh Tg(Col2a1-cre)1Bhr/0	involves: 129S6/SvEvTac * C57BL/6 * SJL