Phenotype Image Detail

Image

Caption

Analysis of apoptosis in the kidneys. A: Kidneys from E14.5 embryos were stained with anti-phospho-histone3 antibody (red) to visualize cells in mitosis and counterstained with cytokeratin (green) and DAPI (blue). No changes in cell proliferation were observed between Lin7c^tm1Dsb/Lin7c⁺ (+/-) and Lin7c^tm1Dsb/Lin7c^tm1Dsb(-/-) kidneys. B: Quantification of the number of mitotic cells. C: Lysotracker (red) was used to label apoptotic cells and counterstained with cytokeratin (green) and DAPI (blue) in embryonic kidneys (E14.5). Mutant kidneys showed a dramatic increase in the number of apoptotic cells. D: Quantification of Lysotracker fluorescence intensity from kidneys. Bar = 100um.

Copyright

This image is from Olsen O, J Cell Biol 2007 Oct 8;179(1):151-64, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:134806

Associated Alleles

Symbol	Name
Lin7c^tm1Dsb	lin-7 homolog C, crumbs cell polarity complex component; targeted mutation 1, David S Bredt

Associated Genotypes

Allelic Composition	Genetic Background
Lin7c^tm1Dsb/Lin7c^tm1Dsb	involves: 129S1/Sv * 129X1/SvJ