Phenotype Image Detail

Image

Caption

A-F: Placenta histology. Genotypes and embryonic stages are indicated. In Ncoa6^tm1Jxu/Ncoa6^tm1Jxu (-/-) placenta, the labyrinth layer (la) is not developed, the maternal blood sinuses (ms) are dilated, and the number of spongiotrophoblast cells (sp) are reduced (compare B with A, D with C, and F with E). A and B were immunostained with BrdUrd antibody and briefly counterstained with hematoxylin. There are more BrdUrd-labeled trophoblast giant cells (gi) and less labeled spongiotrophoblast (sp) and labyrinthine trophoblast (la) cells in mutant placentas (B with A). C and D: Immunostained with PECAM-1 antibody and counterstained with methyl green. E and F were stained with hemotoxylin and eosin. ch, chorionic plate. Bars, 100 um. G-L: Toluidine-stained semithin sections of placentas at 9.75 dpc. G and H compare the three-cell-layered (I,II, and III) epithelial barriers formed by labyrinthine trophoblasts between the maternal blood sinuses (ms) and fetal blood vessels (fv) in wild-type (+/+, G) and mutant (H) placentas. nu, nucleated fetal erythrocytes; me, maternal erythocytes; en, fetal endothelium. I and J: Compare the structure of blood sinuses in wild-type (I) and mutant (J) placentas, showing the loosely attached or detouched syncytiotrophoblasts (arrows) in mutant placentas. K and L show the active erythrophagocytosis (*) of mutant trophoblast cells (K) and trophoblast giant cells (gi in L).

Copyright

This image is from Kuang SQ, J Biol Chem 2002 Nov 22;277(47):45356-60 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:80175

Associated Alleles

Symbol	Name
Ncoa6^tm1Jxu	nuclear receptor coactivator 6; targeted mutation 1, Jianming Xu

Associated Genotypes

Allelic Composition	Genetic Background
Ncoa6^tm1Jxu/Ncoa6^tm1Jxu	involves: 129S6/SvEvTac * C57BL/6