Phenotype Image Detail

Image

Caption

A-C show light microscopy images of the whole-mount preparation of the stria vascularis and underlying spiral ligament. Intermediate cells of the stria vascularis are pigmented cells, and the pigmentation is seen as brown spots all over the stria vascularis. Note the abnormally increased pigment accumulation in the stria vascularis of one- and six-month old S1pr2^tm1Rlp/S1pr2^tm1Rlp (Null) mice. Images were obtained by using a 10x objective and differential interference contrast. Scale bar in A is 100 um and applies to B and C. E-G show degeneration of the organ of Corti in the mutant mouse at postnatal day 31. D, middle turn of the organ of Corti of a wild-type littermate. The rhodamine-phalloidin staining of filamentous actic (red) highlights one row of inner hair cells (IHCs, arrow) and three rows of outer hair cells (OHCs, arrowheads). Arrowheads point to cuticular plates of OHCs with a characteristic V-shape of stereocilia bundles. Cuticular plate of an IHC is indicated by the arrow. The stereocilia of IHCs are present but are not visible, because they are out of the focal plane of this optical section. E, basal turn of the organ of Corti of postnatal day 31 mutant mouse. Arrow points to IHC stereocilia. Arrowheads point to remnants of severely degenerated OHCs in all three rows. Also shown are middle (F) and apical (G) turns of the organ of Corti of the postnatal day 31 mutant mouse. Arrows point to IHC stereocilia. Arrowheads point to the degenerating OHCs. Scale bars in D and G are 10 um. Scale bar in G applies to E and F.

Copyright

This image is from Kono M, J Biol Chem 2007 Apr;282(14):10690-6 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:121165

Associated Alleles

Symbol	Name
S1pr2^tm1Rlp	sphingosine-1-phosphate receptor 2; targeted mutation 1, Richard L Proia

Associated Genotypes

Allelic Composition	Genetic Background
S1pr2^tm1Rlp/S1pr2^tm1Rlp	involves: 129S6/SvEvTac * C57BL/6