Phenotype Image Detail

Image

Caption

Micro-computed tomography of hemimandibles at 7 weeks. Row A, 18-um section through the mandibular first and second molars. The enamel layers of the wild-type (Klk4^+/+) and Klk4^tm1.1Jpsi/Klk4⁺ (Klk4^+/-) mouse molars have similar intensities, which sharply contrast with dentin. The enamel is largely abraded from the molars of the Klk4^tm1.1Jpsi/Klk4^tm1.1Jpsi (Klk4^-/-) mouse. Row B, three-dimensional micro-computed tomography reconstruction of the hemimandibles. Row C, 18-um sections through the mandibular incisor. As in the molars, the enamel layers of the wild-type and heterozygous mouse have similar intensities that sharply contrast with dentin. The incisal edges are sharp and unbroken. The incisor enamel of the homozygous mutant mouse does not contrast well with dentin and is abraded at the incisal edge. Row D, three-dimensional micro-computed tomography reconstruction of the hemimandibles as in row B, but using a threshold cutoff so that only highly mineralized structures are seen. The enamel crowns of the three molars and the incisor in the wild-type hemimandible are uniquely above the threshold. Dentin and bone are both below threshold. The area and volume of the supra-threshold signal (enamel) of the heterogous mouse was reduced by ~10% from the wild-type. In the molar region of the heterozygote, only the thin enamel near the cemento-enamel junction of the first molar appeared to drop below threshold, whereas virtually the entire enamel layer of the molars from the homozygous mutant mouse dropped below threshold.

Copyright

This image is from Simmer JP, J Biol Chem 2009 Jul 10;284(28):19110-21 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:151626

Associated Alleles

Symbol	Name
Klk4^tm1.1Jpsi	kallikrein related-peptidase 4 (prostase, enamel matrix, prostate); targeted mutation 1.1, James P Simmer

Associated Genotypes

Allelic Composition	Genetic Background
Klk4^tm1.1Jpsi/Klk4^tm1.1Jpsi	involves: Black Swiss * C57BL/6