Phenotype Image Detail

Image

Caption

A: Enhanced epidermal hyperplasia in Dgat1^tm1Far/Dgat1^tm1Far (Dgat^-/-) skin after topical retinol treatment. Retinol (50, 100, or 200 nmol dissolved in 100 ul of ethanol or ethanol alone was applied once topically to dorsal skin. Skin was harvested 4 days after treatment, and sections were stained with hematoxylin-eosin (age 6 months, n = 3/genotype/dose). Scale bars, 50 um. B: Increased epidermal thickness in mutant skin treated with retinol. Mice were age 6 months, n = 3/genotype. *, p = 0.0143; **,p = 0.01; ***, p < 0.001 versus vehicle-treated mutant skin. C: Increased susceptibility of mutant skin to retinol-induced irritation. Scaling, cracking, and crusty lesions were prominent in mutant skin. Retinol (100 ul of 1 nmol/ul in ethanol) was applied topically once daily for 3 consective days to the dorsal cephalad skin. Ethanol was applied to the dorsal caudal skin (age 11 weeks, n = 5/genotype).

Copyright

This image is from Shih MY, J Biol Chem 2009 Feb 13;284(7):4292-9 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:147611

Associated Alleles

Symbol	Name
Dgat1^tm1Far	diacylglycerol O-acyltransferase 1; targeted mutation 1, Bob Farese

Associated Genotypes

Allelic Composition	Genetic Background
Dgat1^tm1Far/Dgat1^tm1Far	involves: 129S4/SvJae * C57BL/6J