Gene Expression Image Detail

Reference

Figure

ID

J:122205 Little MH, et al., Gene Expr Patterns. 2007 Jun;7(6):680-99

2

MGI:4849255

Image

Caption

B) Expression of Lim1 in the nephric duct (double arrowhead) and mesonephric glomeruli (arrowhead); Lamb1-1 in the nephric duct (double arrowhead) and mesonephric tubules (arrowhead); Crym in the metanephric mesenchyme (arrow) and mesonephric tubules (double arrowhead); Oct4 in the primordial germ cells (arrow) and Wnt4 in the gonad (arrowhead), mesonephric mesenchyme (double arrowhead), mesonephric tubules and nephric duct, mesonephric portion. Scale bar = 50 microns. (C) Wnt7b: TS20 showed Wnt7b expression in the ureteric trunk (arrowheads) and in the ureter (double arrowheads). Expression was not detected in the ureteric tips. At TS23, the global view shows Wnt7b expression in thecortical collecting ducts (arrowheads) and high magnification of the medulla shows expression in medullary collecting ducts (double arrowhead) and immature loop of Henle (arrowhead). Spint2: Spint2 was expressed in the ureteric tip (double arrowhead) and ureteric trunk (arrowhead) of TS20. At TS23, Spint2 expression continued in the ureteric tip (arrowhead in global image) and cortical and medullary collecting ducts (double arrowhead in high magnification) and was also seen in cortical and medullary renal tubules, including the immature loop of Henle (arrowhead in high magnification). Wnt4: In TS20 Wnt4 was expressed in early nephrons (arrowheads). Later, at TS23, Wnt4 was detected in cortical and medullary collecting ducts and the cortical and medullary interstitium immediately surrounding the collecting ducts (arrowhead in global image TS23) and in the pelvic urothelial lining (TS23). Expression persisted in early nephrons at TS23 with expression in pretubular aggregates, renal vesicles (arrowhead in high magnification), comma-shaped bodies and S-shaped bodies (double arrowheads in high magnification). Col9a1: Col9a1 was expressed in the renal interstitium at all stages (arrowheads). Crym: Crym was strongly and specifically expressed in the cap mesenchyme at all stages presented (arrowheads). Scale bar = 200 microns.

Copyright

Reprinted with permission from Elsevier from doi:10.1016/j.modgep.2007.03.002 Gene Expr Patterns 7: 680-99, Little MH; Brennan J; Georgas K; Davies JA; Davidson DR; Baldock RA; Beverdam A; Bertram JF; Capel B; Chiu HS; Clements D; Cullen-McEwen L; Fleming J; Gilbert T; Herzlinger D; Houghton D; Kaufman MH; Kleymenova E; Koopman PA; Lewis AG; McMahon AP; Mendelsohn CL; Mitchell EK; Rumballe BA; Sweeney DE; Valerius MT; Yamada G; Yang Y; Yu J, A high-resolution anatomical ontology of the developing murine genitourinary tract. Copyright 2007

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
B Lim1	MGI:4849473 (Lhx1)
B Lamb1-1	MGI:4849479 (Lamb1)
B Crym	MGI:4849477 (Crym)
B Oct4	MGI:4849480 (Pou5f1)
B Wnt4	MGI:4849478 (Wnt4)
C Wnt7b TS20	MGI:4849481 (Wnt7b)
C Spint2 TS20	MGI:4849482 (Spint2)
C Wnt4 TS20	MGI:4849478 (Wnt4)
C Col9a1 TS20	MGI:4849483 (Col9a1)
C Crym TS20	MGI:4849477 (Crym)
C Wnt7b TS23 upper	MGI:4849481 (Wnt7b)
C Wnt7b TS23 lower	MGI:4849481 (Wnt7b)
C Spint2 TS23 upper	MGI:4849482 (Spint2)
C Spint2 TS23 lower	MGI:4849482 (Spint2)
C Wnt4 TS23 upper	MGI:4849478 (Wnt4)
C Wnt4 TS23 lower	MGI:4849478 (Wnt4)
C Col9a1 TS23 upper	MGI:4849483 (Col9a1)
C Col9a1 TS23 lower	MGI:4849483 (Col9a1)
C Crym TS23 upper	MGI:4849477 (Crym)
C Crym TS23 lower	MGI:4849477 (Crym)