Phenotype Image Detail

Image

Caption

(A) Sections of wild-type (+/+) and Mapk6^tm1Melo/Mapk6^tm1Melo lungs inflated with fixative were stained with hematoxylin and eosin. Magnification, 5x. Immunostaining for T1alpha (B) and SP-C (C) in lungs from littermate wild-type and mutant embryos at E18.5. Magnification, 40x. (D) Ultrastructure of E18.5 wild-type and mutant lungs. Mature type ll pneumocytes present characteristic villi (black arrow) and contain lamellar bodies (open arrow). Type ll cells from mutant lungs contain an abundant pool of glycogen (*) and present attenuated villi. Secreted surfacant can be observed occasionally in the airspace of mutant embryos (black arrow). (E) PAS staining (arrows) indicating cytoplasmic glycogen in lung sections from E18.5 wild-type and mutant embryos. Magnification, 40x. (F) Quantification of PAS staining (n=5-6). *, P<0.01.

Copyright

This image is from Klinger S, Proc Natl Acad Sci U S A 2009 Sep 29;106(39):16710-5. Copyright 2009 National Academy of Sciences, U.S.A. J:153218

Associated Alleles

Symbol	Name
Mapk6^tm1Melo	mitogen-activated protein kinase 6; targeted mutation 1, Sylvain Meloche

Associated Genotypes

Allelic Composition	Genetic Background
Mapk6^tm1Melo/Mapk6^tm1Melo	involves: 129S1/Sv * 129X1/SvJ * C57BL/6