Phenotype Image Detail

Image

Caption

(A) Body temperature of Lgmn^tm1Ihn/Lgmn^tm1Ihn (filled circles) and Lgmn^tm1Ihn/Lgmn⁺ (open circles) mice at 12 months of age. ***, P<0.005, Student's t test, +/-, n=4, -/-, n=7. Body core temperature was continuously recorded on a potentiometer by a copper-constantan thermocouple covered with polyethylene tubing (outside diameter 1 mm). (B) Age-dependent decrease of circulating hematocrit was seen in homozygous mutant (filled circles) mice but not in the heterozygous control mice (open circles). *, P<0.05, Student's test, 3-month-old: +/-, n=6, -/-, n=6; 6-month-old: +/-, n=7, -/-, n=6; 15-month-old: +/-, n=2, -/-, n=4. (C) Age-dependent increase of circulating reticulocytes was seen in homozygous mutant (filled circles) mice but not in the heterozygous control mice (open circles) *, P<0.05; **, P<0.01, Student's t test. Data were obtained from the same mice examined in B. (D) Circulating Epo from wild-type (filled bars) and homozygous mutant (open bars) were determined by ELISA. A progressive increase in serum Epo was recorded in mutant mice with increased age. *, P<0.05, Student's t test, n=6. (E) Bone marrow sections from femur of wild-type (+/+) and mutant (-/-) mice were stained with EpoR-specific antibody. IHC immunohistochemistry. Enhanced EpoR expression was detected in mutant mice. (Scale bar, 20um).

Copyright

This image is from Chan CB, Proc Natl Acad Sci U S A 2009 Jan 13;106(2):468-73. Copyright 2009 National Academy of Sciences, U.S.A. J:143886

Associated Alleles

Symbol	Name
Lgmn^tm1Ihn	legumain; targeted mutation 1, Ikuko Hara-Nishimura

Associated Genotypes

Allelic Composition	Genetic Background
Lgmn^tm1Ihn/Lgmn^tm1Ihn	involves: 129P2/OlaHsd * C57BL/6