Phenotype Image Detail

Image

Caption

(A) Representative micrographs of H&E stained sectuins of the proximal ends of tibiae from E18.5 wild-type (WT) and Pthlh^tm2Ack/Pthlh^tm2Ack (KI) mice (magnification, x100). Blue lines represent the respective proliferating zones. (B) The length measurements of the proliferating zone. (C) Representative contact radiographs of the femurs of WT and mutant mice at 2 weeks of age. (D) Femoral length measurements. (E) BMD measurements. (F) Representative frontal views of microCT 3D reconstruction of the proximal end of tibiae. (G) Epiphyseal volume of the proximal ends of tibiae. (H) Width of the cartilaginous growth plate. (I) Undecalcified sections of tibiae stained by the von Kossa procedure (magnification, x200). (J) Mineralized area, percentage of growth plate. (K) Paraffin-embedded sections of tibiae from wild-type and mutant mice stained immunohistochemically for PCNA (arrowheads) (magnification, x400). (L) Number of PCNA-positive chondrocytes as a percentage of total chondrocytes, as determined by image analysis. (M) Immunostaining for type X collagen (arrowheads) (magnification, x100). (N) Width of type X collagen-positive hypertrophic zone of growth plates. Data shown represent mean +/- SE of five mice per group. **, P<0.01; ***, P<0.001 in the mutant mice relative to the wild-type mice.

Copyright

This image is from Miao D, Proc Natl Acad Sci U S A 2008 Dec 23;105(51):20309-14. Copyright 2008 National Academy of Sciences, U.S.A. J:142669

Associated Alleles

Symbol	Name
Pthlh^tm2Ack	parathyroid hormone-like peptide; targeted mutation 2, Andrew C Karaplis

Associated Genotypes

Allelic Composition	Genetic Background
Pthlh^tm2Ack/Pthlh^tm2Ack	involves: 129S2/SvPas * C57BL/6