Phenotype Image Detail

Image

Caption

(A) Representative micrographs of hematoxylin and eosin (H&E) stained sections of the metaphyseal region of tibiae from E18.5 wild-type (WT) and Pthlh^tm2Ack/Pthlh^tm2Ack (KI) mice (magnification, x400). (B) Osteoblast number/tissue area (N.Ob/T.Ar). (C) Osteoblast surface/bone surface ratio (Ob.S/BS). (D) Representative longitudinal (top) and cross sections (bottom) of 3D reconstruction of proximal end of tibiae at 2 weeks of age. Quantitative histomorphometry for (E) bone volume/total volume (BV/TV), (F) cortical bone volume (Ct.V), (G) trabecular number (Tb.N), (H) trabecular thickness (Tb.Th), and (I) trabecular separation (Tb.Sp). (J) Representative micrographs of tibial sections from the wild-type and mutant mice stained histochemically for TRAP (magnification, x200). (K) Osteoclast number/tissue area (N.Oc/T.Ar). (L) Representative micrographs of tibial sections from the wild-type and mutant mice stained immunohistochemically for Pth1r (magnification, x400). (M) Pth1r positive area as percent of tissue. (N) Representative micrographs of tibial sections from the wild-type and mutant mice stained with H&E (magnification, x400). (O) Osteoblast number/tissue area (N.Ob/T.Ar). (P) Western blot of long bone extracts for the expression of Cbfa1 (Runx2) and Pth1r. Beta-tubulin was used as a loading control for Western blots. Data shown represent mean +/- SE of five mice per group. ***, P<0.001 in the mutant mice relative to wild-type littermates.

Copyright

This image is from Miao D, Proc Natl Acad Sci U S A 2008 Dec 23;105(51):20309-14. Copyright 2008 National Academy of Sciences, U.S.A. J:142669

Associated Alleles

Symbol	Name
Pthlh^tm2Ack	parathyroid hormone-like peptide; targeted mutation 2, Andrew C Karaplis

Associated Genotypes

Allelic Composition	Genetic Background
Pthlh^tm2Ack/Pthlh^tm2Ack	involves: 129S2/SvPas * C57BL/6