Phenotype Image Detail

Image

Caption

(A) Cornea opacity associated with cornea neovascularization in Lgr4^{Gt(LST020)Byg}/Lgr4^{Gt(LST020)Byg} (-/-) mice. (B) Histological analyses of cornea in mutant mice reveal different types of keratopathy. IF, cornea inflammation and vascularization; CL, cystic-like structure in front of the cornea epithelium; VP, cornea vascular pannus; EP, cornea epithelial plug; LSS, loss of cornea epithelium smoothness. Cornea in wild-type (+/+) mice have no defects. (C) Histological analysis of the cornea epithelium at different developmental stages in mutant and wild-type mice. At P0, mutant mice have similar cornea epithelium morphology compared with wild-type mice. At P10 and day 14 and in 2-month old mice, the cornea epithelium is much thinner and more compact in mutant compared with wild-type mouse corneas. (D) PCNAs staining and Keratin 12 staining are both decreased in the cornea epithelium of mutant mice, indicating defects in proliferation and differentiation. (E) Thin cornea stroma in mutant mice was found in both H&E and Masson's trichrome staining (blue). (F) Disturbed and disorganized fiber structures were found in the cornea of mutant mice by electron microscope. (G) The expression levels of collagen I, collagen V, BGN, OGN, keraton, Den, and lumican were significantly decreased in mutants in the P8 eye. (H) The expression levels of PLOD1 and PLOD2, two key enzymes in collagen homeostasis, are also decreased in mutants compared with wild-type mice.[Scale bars: 85 um (b and d), 43 um (c), and 500 nm (f)].

Copyright

This image is from Weng J, Proc Natl Acad Sci U S A 2008 Apr 22;105(16):6081-6. Copyright 2008 National Academy of Sciences, U.S.A. J:134299

Associated Alleles

Symbol	Name
Lgr4^Gt(LST020)Byg	leucine-rich repeat-containing G protein-coupled receptor 4; gene trap LST020, BayGenomics

Associated Genotypes

Allelic Composition	Genetic Background
Lgr4^Gt(LST020)Byg/Lgr4^Gt(LST020)Byg	involves: 129P2/OlaHsd * C57BL/6