Phenotype Image Detail

Image

Caption

(A) Noct^tm1Bjc/Noct^tm1Bjc (Noc -/-) have decreased lipid accumulation in livers. Livers were stained with ORO, and the area fraction of ORO staining was determined in liver chords surrounding portal and central veins separately by using size-calibrated images and Image J software. The values represent group means (+/-SEM) of sections from six mice per genotype [t test: P=0.0035 for portal area; not significant (P=0.06) for central area]. (B) Body weights of male wild-type (WT) (filled symbols) and mutant (open symbols) mice from 4 to 13 weeks ad libitum access to the standard diet were not different (WT, n=24; mutant, n=36; analysis of covariance: F 1,59=0.1222, P=0.728). (C) Male wild-type mice (filled symbols) gained more weight than male mutant mice (open symbols) when given ad libitum access to a high-fat diet (wild-type, n=8; mutant, n=12) for 14 weeks beginning at 8 weeks of age (repeated-measures ANOVA: P=0.042). Values are group means +/- SEM. (D) Photograph of age-matched wild-type and mutant mice showing body size differences after 20 weeks on the high-fat diet. (E) Mutant livers at 20 weeks did not accumulate excess fat on the high-fat diet. Greater accumulation of lipid in wild-type (left) compared with mutant (right) livers at the macroscopic level (upper) and in ORO-stained sections (lower) was observed. (F) Epididymal fat pads dissected at ZT1 from mutant mice (open bars) on a high-fat diet are significantly smaller than those of wild-type controls (filled bars). Shown are average weights (+/- SEM) of one epididymal fat pad from each mouse [for the standard diet, n=5 for both genotypes (t test: P=0.17); for the high-fat diet, n=5 mutant and n=4 wild-type (t test: P=0.024)].

Copyright

This image is from Green CB, Proc Natl Acad Sci U S A 2007 Jun 5;104(23):9888-93. Copyright 2007 National Academy of Sciences, U.S.A. J:122301

Associated Alleles

Symbol	Name
Noct^tm1Bjc	nocturnin; targeted mutation 1, J C Besharse

Associated Genotypes

Allelic Composition	Genetic Background
Noct^tm1Bjc/Noct^tm1Bjc	B6.129S2-Noct^tm1Bjc