Phenotype Image Detail

Image

Caption

Cellular proliferation and apoptosis in Gata4^tm1Jml/Gata4⁺ Gata5^tm1Eem/ Gata5^tm1Eem hearts. Cardiac myocytes in Gata4^tm1Jml/Gata4⁺ Gata5^tm1Eem/ Gata5^tm1Eem (Gata4^+/- 5^-/-) compound mutants (B and D) demonstrate a 51% (at E12.5) and 55% (at E14.5) reduced staining with either Ki67 or phosphohistone H3 (red) compared with wild-type (WT) embryos (A,C and summarized in E), MF20 (green), and 4',6-diamidino-2-phenylindole (blue). Similar decreases in proliferation in compound mutants results were observed by immunohistochemistry analysis with phosphohistone H3 (pH-H3, F). There was no change in apoptosis with respect to WT embryos in compound mutants as assessed by TUNEL stain (G). *,p<0.05.

Copyright

This image is from Singh MK, J Biol Chem 2010 Jan 15;285(3):1765-72 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:155855

Associated Alleles

Symbol	Name
Gata4^tm1Jml	GATA binding protein 4; targeted mutation 1, Jeffrey M Leiden
Gata5^tm1Eem	GATA binding protein 5; targeted mutation 1, Edward E Morrisey

Associated Genotypes

Allelic Composition	Genetic Background
Gata4^tm1Jml/Gata4^+ Gata5^tm1Eem/Gata5^tm1Eem	involves: 129S1/Sv * 129X1/SvJ * C57BL/6