Phenotype Image Detail

Image

Caption

Primary inner limiting membrane components are present, but abnormalities are evident by light and electron microscopy of Lama1^nmf223/Lama1^nmf223 mice. Immunohistochemical labeling of littermate wild-type (A,C,E,G and I) and Lama1^nmf223/Lama1^nmf223(B,D,F,H and J) retinas with anti-laminin alpha1, anti-collagen IV, anti-perlecan, anti-dystroglycan, and anti-integrin beta1 is shown with retinal layers defined as inner limiting membrane (ILM) inner nuclear layer (INL), and outer nuclear layer (ONL). Immunohistochemical staining for anti-glutamine sythetase (GS) demonstrates that Muller cell end-feet reach the inner limiting membrane in both littermate wild-type (K) and mutants (L). H&E staining of wild-type (M) and mutant (N) retinas shows ectopic vessels and cells within the vitreous of mutants. Electron microscopy confirms the inner limiting membrane disruptions. The inner limiting membrane of wild-type (O) mice is continuous above the ganglion cell (GC) layer, whereas the mutants exhibit frequent breaks through which Muller cells (MC) protruded (P). The arrows indicate the inner limiting membrane. Scale bars, 10um (A-N) and 1um (O and P).

Copyright

This image is from Edwards MM, J Biol Chem 2010 Mar 5;285(10):7697-711 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:160722

Associated Alleles

Symbol	Name
Lama1^nmf223	laminin, alpha 1; neuroscience mutagenesis facility, 223

Associated Genotypes

Allelic Composition	Genetic Background
Lama1^nmf223/Lama1^nmf223	C57BL/6J-Lama1^nmf223/J