Phenotype Image Detail

Image

Caption

Astrocyte and blood vessel development are altered in Lama1^nmf223/Lama1^nmf223 mice. Immunohistochemical staining with anti-GFAP and G. simplicifolia isolectin (GS isolectin) in P0.5-P1 central retina of wild-type (A) and Lama1^nmf223/Lama1^nmf223 (B) mice demonstrates the apparent lack of retinal vessels in mutants. P7 central retina of wild-type (C) and mutants (D) stained with anti-GFAP and G. simplicifolia isolectin. ADPase staining of P7 retinas shows a complete primary plexus as well as the formation of capillaries in wild-type retinas (E), whereas in the mutant retina, budding of the vitreal vessels is observed, but no capillaries are present (F). The arrows indicate the hyaloid remnants in wild-type mice and budding hyaloid vessels in mutants, whereas the asterisks indicate areas devoid of retinal vessels in mutants. Labeling with anti-endostatin and G. simplicifolia isolectin at P10 demonstrates the failure of hyaloid vessels to regress in mutants (H) compared with wild-type mice (G). Only endostatin-positive primary-like vessels aer observed in mutant retinas. The arrow in G indicates the hyaloid artery in wild-type mice, whereas circles in H highlight anastomoses of hyaloid vessels entering the retina. Rather than within the retina, as seen in controls (I), in adult retinas, ADPase staining confirms that primary-like vessels lie in the vitreous of mutants (J).

Copyright

This image is from Edwards MM, J Biol Chem 2010 Mar 5;285(10):7697-711 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:160722

Associated Alleles

Symbol	Name
Lama1^nmf223	laminin, alpha 1; neuroscience mutagenesis facility, 223

Associated Genotypes

Allelic Composition	Genetic Background
Lama1^nmf223/Lama1^nmf223	C57BL/6J-Lama1^nmf223/J