Phenotype Image Detail

Image

Caption

Shown is immunofluorescence analysis of 4 week-old eyes from littermate controls (A,C,E and G) and Lama1^tm1Olf/Lama1^tm1Olf Edil3^{Tg(Sox2-cre)1Amc}/0 mice (Lama1^delta, B,D,F and H) with anti-laminin alpha1, anti-collagen IV, anti-perlecan, and anti-dystroglycan antibodies. Histological examination with H/E staining demonstrates that the well organized ganglion cell layer and intact inner limiting membrane seen in controls (I) are replaced by a disorderly ganglion cell layer with ectopic cells and vessels within the vitreous in mutants (J). Glutamine synthetase staining shows that, in the control retinas, Muller cell end-feet lie at the inner limiting membrance (K). In the mutant retinas, however, Muller cell processes fall short, terminating in the inner plexiform layer, and are lacking end-feet (L). Ectopic cells (arrows) are also visible on the vitreal side of the retina in mutant retinas.

Copyright

This image is from Edwards MM, J Biol Chem 2010 Mar 5;285(10):7697-711 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:160722

Associated Alleles

Symbol	Name
Edil3^Tg(Sox2-cre)1Amc	EGF-like repeats and discoidin I-like domains 3; transgene insertion 1, Andrew P McMahon
Lama1^tm1Olf	laminin, alpha 1; targeted mutation 1, Olivier Lefebvre

Associated Genotypes

Allelic Composition	Genetic Background
Lama1^tm1Olf/Lama1^tm1Olf Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129S2/SvPas * C57BL/6J * CBA * CD-1 * Swiss Webster