Phenotype Image Detail

Image

Caption

A and B, antibodies to neuronal specific nuclear protein (NeuN) were used to visualize neuronal cell bodies in all brain regions. A and B, representative photomicrographs of the premotor cortex are shown for wild-type (wt) (A) and Canx^{Gt(KST286)Byg}/Canx^{Gt(KST286)Byg} (cnx-/-) (B). Normal cortical cytoarchitecture was observed, and neurons appeared normal and healthy (arrows point to NeuN positive neurons). Scale bar, 50 um. Inset, scale bar, 10 um. C and D, examination of large white matter tracts showed that the rostral corpus callosum was thinner and contained patchy areas of myelination in the absence of calnexin (D). The asterisks in C and D identify rostral corpus callosum and areas magnified for insets. Scale bar, 50 um. Inset, scale bar, 20 um. E and F, compared with wild-type (E), the overall amount of myelin in the internal capsule (identified with arrows) was reduced in mutant mice (F). Scale bar, 50 um. G and H, the cerebral peduncle (identified with asterisks) in the mutant mouse (H) contained areas of patchy myelin compared with wild-type (G). Scale bar, 50 um. I and J, the cerebellar peduncles (identified with arrows) in the mutant mouse (J) showed a patchy, loose myelination pattern as compared with wild-type animals (I). Scale bar, 20 um. K and L, in the white matter of the spinal cord, an increased number of glial fibrillary acid protein-positive astrocytic fibers (identified with asterisks) was observed in the absence of calnexin (L) compared with the wild-type (K). Scale bar, 10 um. Three sets of 6-8-week-old wild-type and mutant mice were examined.

Copyright

This image is from Kraus A, J Biol Chem 2010 Jun 11;285(24):18928-38 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:164553

Associated Alleles

Symbol	Name
Canx^Gt(KST286)Byg	calnexin; gene trap KST286, BayGenomics

Associated Genotypes

Allelic Composition	Genetic Background
Canx^Gt(KST286)Byg/Canx^Gt(KST286)Byg	involves: 129P2/OlaHsd * C57BL/6