Phenotype Image Detail

Image

Caption

Defects in mucous gland cell maturation in Spdef^tm1Shiv/Spdef^tm1Shiv antra. A, significant (p < 0.001) decrease of Muc6 and Tff2 mRNAs in Spdef^tm1Shiv/Spdef^tm1Shiv (KO) antra as compared with the wild-type (WT), as determined by quantitative RT-PCR. B and C, strongly reduced staining for Tff2 (B) and GS-II lectin (C) in mutant antral mucous gland cells as compared with WT littermates. D and E, significantly reduced numbers of secretory granules in antral mucous gland cells of mutant mice as compared with WT littermates. Granule counts (E) show data from three independent mouse pairs. F, immature appearance of granules in mutant antral mucous gland cells, with common absence of the WT feature of a composite of electron-dense and less dense compartments. Scale bars, B and C, 50 um; D, 2 um; and F, 0.5 um. Error bars in A and E indicate S.E.

Copyright

This image is from Horst D, J Biol Chem 2010 Nov 5;285(45):35047-55 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:166876

Associated Alleles

Symbol	Name
Spdef^tm1Shiv	SAM pointed domain containing ets transcription factor; targeted mutation 1, Ramesh Shivdasani

Associated Genotypes

Allelic Composition	Genetic Background
Spdef^tm1Shiv/Spdef^tm1Shiv	involves: 129S4/SvJae