Phenotype Image Detail

Image

Caption

(A) Growth retardation of Naca^tm1Dsr/Naca^tm1Dsr (skNAC^-/-) mice at 1 wk (P7) and 5 months of age. (B) Body weight of mutant mice and gender-matched littermates at 3 months of age (male, n = 6; female, n - 11). The average is marked with a black line (*P<0.01). (C) Reduced muscle mass in mutant mice. (i and ii) A comparison of H&E-stained sections of wild-type and mutant calf muscles shows the smaller size of the mutant muscle at 3 months of age. (iii and iv) At higher magnification, cross-sectional area of individual myofibers looks similar in wild-type and mutant soleus. (Scale bars, 100 um.) (D and E) Quantitative analysis of myofibers in mutant or wild-type muscle. The number of myofibers in 480,000 um² was counted, and the cross-sectional area of individual fibers was measured by using ImageJ software. Data are presented as mean with SE (n = 3). (F) Muscle fiber type markers in soleus of mutant mice. qPCR revealed that expression of Myh4 (glycolytic, type llb) was significantly increased in mutant muscle, whereas Myh7 (oxidative, type I) and Myh2 (oxidative, type lla) markers were reduced. Data are presented as mean with SEs (n = 3, *P < 0.05, **P < 0.01). (G) Histochemical assay for SDH activity (blue stain) to measure oxidative potential in mutant soleus muscle. The mutant soleus demonstrated less oxidative potential than wild-type soleus. Representative samples are shown (n = 3).

Copyright

This image is from Park CY, Proc Natl Acad Sci U S A 2010 Nov 30;107(48):20750-5. Copyright 2010 National Academy of Sciences, U.S.A. J:167169

Associated Alleles

Symbol	Name
Naca^tm1Dsr	nascent polypeptide-associated complex alpha polypeptide; targeted mutation 1, Deepak Srivastava

Associated Genotypes

Allelic Composition	Genetic Background
Naca^tm1Dsr/Naca^tm1Dsr	129S6/SvEvTac-Naca^tm1Dsr