Phenotype Image Detail

Image

Caption

Regeneration defects of skeletal muscle in Naca^tm1Dsr/Naca^tm1Dsr mice. (A) Hemotoxylin & eosin staining of skeletal muscle 12 days after cardiotoxin-induced injury. Note normalizing architecture in wild-type muscle, with regenerating myofibers containing centrally located nuclei (arrowhead). Naca^tm1Dsr/Naca^tm1Dsr (skNAC^-/-) muscle had signs of myonecrosis, with infiltrating mononuclear cells (arrow) and fat deposits (asterisk). Accumulation of fat in regenerating myofibers in mutant mice was marked by Oil Red O staining. (Scale bars, 100 um.). (B) Mutant muscle still had many myofibers with central nuclei (arrowhead), mononuclear infiltrating cells (arrow), and adipocytes (asterisk) 1 month after injury; wild-type muscle was almost indistinguishable from preinjury muscle. Oil Red O staining indicates fat replacement. (Scale bars, 100 um.) (C) Quantitative analysis of regenerating myofibers with central nuclei 12 days after the injury (n = 3). (D) Quantitative analysis of cross-sectional area of regenerated myofibers 1 month after the injury. Myofibers of mutant mice had smaller cross-sectional area than wild-type myofibers (*P < 0.0001). (E and F) Proliferation and cell death profiles of satellite cells isolated from mutant mice. Single satellite cells isolated from the tibialis anterior muscle of mutant or control (skNAC^+/-) animals were plated within a hydrogel-microwell array and monitored for 96 hours by time-lapse microscopy. A representative graph from the three experimental replicates is displayed. Data are shown as mean with SE (*P < 0.05). (E) There was no significant difference in proliferation profiles of mutant and mutant satellite cells 96 hours after plating. (F) Satellite cells from mutant mice had a higher cell death percentage than those from control mutant littermates over the 96-hour period.

Copyright

This image is from Park CY, Proc Natl Acad Sci U S A 2010 Nov 30;107(48):20750-5. Copyright 2010 National Academy of Sciences, U.S.A. J:167169

Associated Alleles

Symbol	Name
Naca^tm1Dsr	nascent polypeptide-associated complex alpha polypeptide; targeted mutation 1, Deepak Srivastava

Associated Genotypes

Allelic Composition	Genetic Background
Naca^tm1Dsr/Naca^tm1Dsr	129S6/SvEvTac-Naca^tm1Dsr