Phenotype Image Detail

Image

Caption

(A) The gene-targeting strategy for Tdrd5^tm1Sait/Tdrd5^tm1Sait (Tdrd5). The correct targeting generates ~7.0-kb bands when digested with EcoRV and probed with the 5' probe (green boxes). (B) Southern blot for the detection of the gene targeting. (C and D) Northern (C) or Western (D) blots of wild-type and mutant testes for mutant or TDRD5 expression. (C) A, probe A (5'); B, probe B (3') as shown in Fig. 1A. T5-1, -2, and -3, Tdrd5 isoforms 1, 2, and 3. (E) Testes of wild-type and mutant mice at 4- and 8-weeks of age. (F) The weight (the averages with SD; error bars) of wild-type and mutant testes (8 week old). (G) Histology of wild-type and mutant testes (top) and epididymis (bottom) stained by hematoxylin and eosin. (H) Alkaline phosphatase staining of wild-type and mutant male (left) and female (right) gonads at E12.5. Bars: (E) 2 mm; (G) 50 um; (H) 300 um.

Copyright

This image is from Yabuta Y, J Cell Biol 2011 Mar 7;192(5):781-95, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:170319

Associated Alleles

Symbol	Name
Tdrd5^tm1Sait	tudor domain containing 5; targeted mutation 1, Mitinori Saitou

Associated Genotypes

Allelic Composition	Genetic Background
Tdrd5^tm1Sait/Tdrd5^tm1Sait	involves: C57BL/6 * CBA